recombinant human fgf-basic (154 aa) (250) Search Results


fgf2  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc fgf2
A L6-WT, L6-V561M, H1581-WT, and H1581-V561M cells were starved for 12 h, then stimulated with 50 ng/mL <t>FGF2</t> and 10 μg/mL heparin for 0, 1, 5, or 15 minutes before lysis and harvesting. 5 μg of protein was loaded onto an SDS-PAGE gel, transferred to a nitrocellulose membrane, then blotted for total and phospho-protein levels of FGFR and various signaling partners. B pSTAT3 levels for three replicate immunoblots of L6 and H1581 WT and V561M cells stimulated with 50 ng/mL FGF2 and 10 μg/mL heparin for 5 minutes were quantified using ImageJ and plotted using GraphPad Prism. C Basal L6-WT, L6-V561M, H1581-WT and H1581-V561M cells were harvested and lysed, and immunoblots were performed as described for 2A. D pSTAT3 and STAT3 gene target expression in L6- and E H1581- WT and V561M cell lines were normalized against loading controls and quantified using ImageJ. Data were plotted using GraphPad Prism. Significance values: *<0.05; **<0.01, ***<0.001.
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A L6-WT, L6-V561M, H1581-WT, and H1581-V561M cells were starved for 12 h, then stimulated with 50 ng/mL FGF2 and 10 μg/mL heparin for 0, 1, 5, or 15 minutes before lysis and harvesting. 5 μg of protein was loaded onto an SDS-PAGE gel, transferred to a nitrocellulose membrane, then blotted for total and phospho-protein levels of FGFR and various signaling partners. B pSTAT3 levels for three replicate immunoblots of L6 and H1581 WT and V561M cells stimulated with 50 ng/mL FGF2 and 10 μg/mL heparin for 5 minutes were quantified using ImageJ and plotted using GraphPad Prism. C Basal L6-WT, L6-V561M, H1581-WT and H1581-V561M cells were harvested and lysed, and immunoblots were performed as described for 2A. D pSTAT3 and STAT3 gene target expression in L6- and E H1581- WT and V561M cell lines were normalized against loading controls and quantified using ImageJ. Data were plotted using GraphPad Prism. Significance values: *<0.05; **<0.01, ***<0.001.

Journal: Molecular cancer research : MCR

Article Title: The FGFR1 V561M Gatekeeper Mutation Drives AZD4547 Resistance through STAT3 Activation and EMT

doi: 10.1158/1541-7786.MCR-18-0429

Figure Lengend Snippet: A L6-WT, L6-V561M, H1581-WT, and H1581-V561M cells were starved for 12 h, then stimulated with 50 ng/mL FGF2 and 10 μg/mL heparin for 0, 1, 5, or 15 minutes before lysis and harvesting. 5 μg of protein was loaded onto an SDS-PAGE gel, transferred to a nitrocellulose membrane, then blotted for total and phospho-protein levels of FGFR and various signaling partners. B pSTAT3 levels for three replicate immunoblots of L6 and H1581 WT and V561M cells stimulated with 50 ng/mL FGF2 and 10 μg/mL heparin for 5 minutes were quantified using ImageJ and plotted using GraphPad Prism. C Basal L6-WT, L6-V561M, H1581-WT and H1581-V561M cells were harvested and lysed, and immunoblots were performed as described for 2A. D pSTAT3 and STAT3 gene target expression in L6- and E H1581- WT and V561M cell lines were normalized against loading controls and quantified using ImageJ. Data were plotted using GraphPad Prism. Significance values: *<0.05; **<0.01, ***<0.001.

Article Snippet: The following antibodies were used (all primary antibodies are rabbit derived unless otherwise noted): anti-FGFR1 (#9740), phospho-FGFR (Y653/654, #3476, mouse derived), phospho-FRS2 (Y436, #3861; Y196, #3864), PLCγ (#5690), phospho-PLCγ (Y783, #2821), ERK (#4695), phospho-ERK (T202/Y204 #4370), Akt (#4691), phospho-Akt (S473 #4060), STAT3 (#4904), phospho-STAT3 (Y705, #9145), E-cadherin (#3195), Vimentin (#5741), ZEB1 (#3396), N-cadherin (#13116), HIF1α (#14179), Bcl-XL (#2764), Hsp90 (#4877), FGF2 (#61977), TGFβ (#3711), Cyclin D1 (#2978), GAPDH (#2118), horseradish peroxidase (HRP)-linked rabbit IgG secondary antibody (#7074) and HRP-linked mouse IgG secondary antibody (#7076), from Cell Signaling Technology.

Techniques: Lysis, SDS Page, Membrane, Western Blot, Expressing